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Multi-Channel Images

Montaris-X has full support for multi-channel scientific images — common in fluorescence microscopy, immunohistochemistry, and multi-spectral imaging.

Opening Multi-Channel Images​

When you open a multi-channel TIFF, Montaris-X detects the channels automatically and presents a Montage Document dialog:

  • Montage view: All channels arranged in a grid
  • Single channel: View one channel at a time
  • Downsample dialog: For very large images, choose a working resolution

Composite Display​

View multiple channels overlaid with distinct color tints:

  • Each channel gets its own tint color (e.g., DAPI = blue, GFP = green, RFP = red)
  • Adjust individual channel visibility
  • The composite updates in real time as you toggle channels

False Color​

Apply false-color lookup tables to single-channel images:

  • Switch between grayscale and false-color in the Display Panel
  • Useful for visualizing intensity variations in 16/32-bit data

Channel Tints​

Customize the color assigned to each channel:

  • Click the color swatch next to a channel in the Display Panel
  • Choose from preset fluorophore colors or pick a custom color
  • Tints are applied in real time to the composite view

Document Switching​

When working with multiple montage documents:

  • Switch between documents using the document tabs
  • Each document maintains its own ROI layers, zoom level, and display settings
  • ROIs are independent per document

Downsampling​

For very large multi-channel images:

  • A dialog prompts you to choose a downsample factor on load
  • Work at reduced resolution for faster navigation
  • Exported masks are automatically upscaled to the original resolution